Expression, purification, and bioassay of human stanniocalcin from baculovirus-infected insect cells and recombinant CHO cells.

Lay Summary

This study successfully produced and tested human stanniocalcin (hSTC), a hormone known for regulating calcium and phosphate, from both insect cells infected with baculovirus and Chinese hamster ovary (CHO) cells. The research focused on cloning the hSTC gene, expressing it in these cells, and purifying the hormone. Importantly, the study examined how the hormone’s glycosylation (the process of adding sugar molecules to proteins) affected its function. Through bioassays in fish, it was found that hSTC from both sources was biologically active, influencing calcium transport in fish gills in a dose-dependent manner. Despite differences in glycosylation, the hSTC produced from both cell types showed similar potency, suggesting glycosylation differences did not significantly impact the hormone’s biological activity in these tests. This research contributes to understanding how hSTC works and paves the way for further studies on its role in mammals.

Reference

Zhang, J., Alfonso, P., Thotakura, N. R., Su, J., Buergin, M., Parmelee, D., … & Gentz, R. (1998). Expression, purification, and bioassay of human stanniocalcin from baculovirus-infected insect cells and recombinant CHO cells. Protein expression and purification, 12(3), 390-398.

DOI: https://doi.org/10.1006/prep.1997.0857